Why Choosing a HPLC Column Supplier is Important
Having the right HPLC column for your application is crucial to the success of your analysis. When buying a new HPLC column make sure that it has been designed to suit your specific requirements and can handle the conditions that you will be putting it through.
Using a wrong HPLC column will have detrimental effects on your results, and can even cause you to waste valuable time trying to get it working correctly. The best way to avoid these problems is to purchase a column from a reputable supplier.
The HPLC system is a combination of a solvent reservoir, pump, injector, column and detector. It is used to separate different components of a sample by liquid chromatography. It can also be used to measure the concentration of various components in the sample.
A hplc column supplier will also help you to identify your needs and select the appropriate HPLC column for each specific application. This will save you time and money in the long run, as well as give you the confidence that your HPLC system is able to perform the analysis that you require.
Your hplc column is the heart of your system and must be correctly installed for it to work properly. It should be set up for the correct solvents, pH, temperature, pressure and % organic/aqueous to ensure that your sample will not be contaminated or lose its purity.
If your HPLC system is not correctly set up or if the wrong solvents are used you will likely experience issues with high pressure, peak tailing, carryover, ghost peaks and wider peaks that are difficult to interpret. You should also note the equilibration times and the number of injections that the HPLC column has to be re-equilibrated after each run.
One way to keep on top of your column is to use a logging system and record the date that it was purchased, its name, type of analysis and the type of solvents used. This information will help you to identify any potential problems and make any necessary adjustments.
Keeping a test chromatogram of your new column can also be helpful when it comes to understanding how well it is performing. This will show you any changes in resolution or chromatogram quality that may occur over time due to interactions with your mobile phase, analytes and sample matrices.
The latest HPLC column technology should be checked by a qualified laboratory technician on a regular basis to ensure that it is operating safely and efficiently. You should also take note of any problems such as high pressure, peak tailing or carrying over that you notice and take action to rectify them.
To protect your hplc column from damage and improve its performance it is essential to clean it regularly. This is a simple procedure and should be repeated before each analysis to ensure that your HPLC system will be running at its best. Check out this post: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography for more details related to this article.